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engineering
natural bioflocculants: production, application, and mechanisms

Type of project: MSc Thesis/Internship
Anticipated start: from April 2025, a later starting time is possible
Duration: > 6 months
Location: Wetsus, European centre of excellence for sustainable water technology, Leeuwarden, the Netherlands
Allowance: The allowance for students is €200. The student can apply for the ‘Wetsus Grant’ allowance (€200 extra; an internal committee decides if the allowance will be granted).

Project Background
Synthetic flocculants such as polyacrylamide are widely used in (waste)water treatment due to their good flocculation capacity. The great flocculation performance of these synthetic flocculants are mainly due to their properties like high molecular weight and charge density. However, these synthetic flocculants derived from fossil fuels lead to environmental issues like a high carbon footprint and eco-toxicity. Extracellular polymeric substances (EPS), biodegradable microbially-secreted polymers in wastewater treatment, have similar properties to synthetic flocculants. Therefore, biodegradable EPS are considered a green alternative in flocculation.

The flocculation capacity of EPS is influenced by their properties like molecular weight, charge density, and branching degree. However, how to regulate EPS properties to enhance their flocculation performance is still unknown, especially in the mixed culture. Understanding the mechanisms behind EPS production in the mixed culture is crucial for producing EPS with improved flocculation capacity.

The goal of this project is to produce engineered EPS with specific properties from wastewater, and understand the mechanisms of engineered EPS production. The outcome of this project is expected to promote EPS to outperform synthetic flocculants and contribute to sustainability in (waste)water treatment.

 

Your task

  • Operate bioreactors under different conditions
  • Water quality analysis (COD, TN, TP, and MLSS)
  • Kinetic experiment of EPS production
  • Extract EPS and characterize EPS (molecular weight, charge density, radius of gyration, functional groups)
  • Test EPS flocculation performance

 

Your profile

  • Background in Environmental Engineering/Chemical Engineering/Biotechnology, or related fields;
  • Great interest in bioprocesses, wastewater treatment and biopolymers;
  • Highly motivated, enthusiastic, and willing to learn about bioprocess and flocculation;
  • Fluent in English and good English writing skills;
  • It would be a plus if you have experience with bioreactor and modeling.

How to apply
Please upload your CV (max. 2 pages, A4 page), transcript (combine with your CV in one file), and motivation letter (max. 1 page, A4 page) (in English). You can contact Bohan Chen (bohan.chen@wetsus.nl) if you have any questions or need more information.

Application form: Engineering natural bioflocculants: production, application, and mechanisms

  • Max. bestandsgrootte: 1 GB.
  • Max. bestandsgrootte: 1 GB.

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